ESTERIFICATION OF OLEIC ACID AND ETHYL ALCOHOL FOR SYNTHESIS OF ETHYL OLEATE CATALYZED BY LIPASE IMMOBILIZED IN POLYURETHANE
Palavras-chave:
Candida antarctica, Lipase B. Ester synthesis, Isoamyl acetate, Biocatalysis.Resumo
Esters are one of the most important classes of organic compounds that are synthesized by various mechanisms, including the reaction between an alcohol and carboxylic acid, with the elimination of water, called esterification. Accordingly, the purpose of this study was to evaluate the production of ethyl oleate by esterification of oleic acid with ethanol in the presence of lipase from Candida antarctica B free and immobilized in polyurethane subjected to a mechanical and ultrasonic system and organic solvent free. The experiments for the synthesis of ethyl oleate were conducted by varying the temperature (°C) and mass catalyst (g). The maximization of esterification results is obtained for the free enzyme after 25 minutes (688.24 U g-1). For polyurethane immobilized enzyme after 40 minutes of mechanical stirring reaction system was reached 1553.83 U g-1 and 19 cycles of reuse. Considering the ultrasonic agitation system after 15 minutes of reaction was reached 3087.60 U g-1 and 14 cycles of reuse. From the obtained results, we can consider that the established process was efficient, mainly in relation to the significant reduction of the reaction time, with low instrumental requirements employed and the improvement of the general performance of the bioprocess, through the results obtained in terms of esterification. In addition, the established process can be considered an environmentally friendly and economically viable technology, and can be used in cosmetics, pharmaceuticals, biodiesel and food industry.
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