Nádia Ligianara Dewes Nyari, Paulazzi Rogério Alessandro, Raquel Vera Zamadei, Jamile Zeni, Rogério Marcos Dallago


The Candida antarctica lipase B immobilized on polyurethane catalysed esterification of oleic acid with ethyl alcohol in mechanical and ultrasonic agitation system in a system solvent-free organic, was studied. The maximum esterification of ethyl oleate was 1553.83 U/g with 19 cycles of reuse after 40 minutes of reaction time in mechanical system and 3087.60 U/g was observed and 14 cycles de reuse after 15 minutes of reaction time in ultrasound system. Thus, the process was considered efficient with significant reduction of the reaction time, low instrumental requirements and improve the bioprocess performance. Until now, there were no studies available in the open literature in relation to the ester synthesis catalyzed by immobilized lipase in polyurethane as support in the ultrasound system, especially in terms of esterification activity. In addition, considered an environmentally correct and economically viable technology, it can be used in cosmetics, pharmaceuticals, biodiesel and food industry.


Candida antarctica lipase B, ester synthesis, isoamyl acetate, biocatalysis.

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